Abstract
For the last ten years, quantum dots modified by biological materials have made it possible to study biochemical processes by means of biomedical imaging. This thesis introduced how the fluorescence CdTe quantum dots/hydroxyapatite composites were synthesized and how their structure, morphology, and fluorescence property were characterized by using TEM, XRD, EDS, UV-vis absorption spectra, and fluorescence spectra. The fluorescence spectra indicated the superb photometric characteristics of CdTe/HA composites. We also found that refluxing temperature and time had prominent effects on fluorescence wavelength and intensity of CdTe/HA composites, so the fluorescence emission wavelength of CdTe/HA composites could be controlled. In addition, the effect of BSA on the fluorescence properties of CdTe/HA composites was studied. The fluorescent emission intensity of CdTe/HA composites was enhanced directly with increasing concentrations of BSA; meanwhile, the fluorescence emission intensity of BSA dramatically decreased, which indicated that a Förster nonradiative energy transfer process occurred through the formation of chemical bonds between BSA and CdTe/HA composites. And the two-dimensional correlation (2D COS) was used to analyze the BSA solution before and after the reaction, which indicated that CdTe/HA composites have bound to a site at the surface of the molecule in the first subdomain IA. We also found that there was a linear relationship between the fluorescence intensity enhancement (F/F0) of CdTe/HA composites and the concentration of the bovine serum albumin, which might become a method for quantitative analysis of BSA in a real sample.
Highlights
The fluorescent properties of biocompatible nanoparticles have offered attractive possibilities for medical purposes and multifunctional biological imaging to understand the biochemical processes in vitro and in vivo
The fluorescence intensity of CdTe/HA composites did not decrease as the temperature increased, which indicated that the CdTe quantum dots and HA were not simple physical adsorption but surface complexation and ion exchange adsorption through the formation of stable chemical bond
According to the prediction of the Forster nonradiative energy transfer theory, these results indicate that the energy transfer between Bovine serum albumin (BSA) and CdTe/HA composites could occur with high probability, resulting in the fluorescence quenching of BSA and fluorescence enhancement of CdTe/HA composites
Summary
The fluorescent properties of biocompatible nanoparticles have offered attractive possibilities for medical purposes (drug and gene delivery applications) and multifunctional biological imaging to understand the biochemical processes in vitro and in vivo. The N-acetylL-cysteine-capped CdSe-polyelectrolytes @ hydroxyapatite composite microspheres had been applied in detecting copper ion based on the fluorescence quenching of CdSe. To date, the quantum dots sensors functioned mainly through fluorescence quenching [30,31,32,33,34,35], and the synthesis of these materials with fluorescence enhancement signal has become a new challenge. The quantum dots sensors functioned mainly through fluorescence quenching [30,31,32,33,34,35], and the synthesis of these materials with fluorescence enhancement signal has become a new challenge In this aspect, the coprecipitation method was used to synthesize CdTe/HA composites, and its product has bright fluorescence emission and good biocompatibility. The fluorescent emission intensity of CdTe/HA composites (F/F0) was enhanced linearly with increasing concentration of BSA, which suggested its great potential in real sample detection
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