Abstract
AbstractA process was devised for the synthesis of tris(hydroxymethyl)acetic acid by means of bio‐oxidation of pentaerythritol. A flavobacterium able to grow on pentaerythritol was isolated from the soil. Mutants were obtained that, were deficient, in their pentaerythritol oxidation system. These mutants could not grow on pentaerythritol, but when grown on an assimilable carbon source; they oxidized pentaerythritol to tris(hydroxymethyl)acetic acid. This conversion readily took place in a medium consisting of 20 g pentaerythritol, 10 g yeast extract, and 2 g acetic acid (neutralized) per liter. Since the mutants were unable to metabolize tris(hydroxymethyl)acetic acid, theoretical conversion yields were attainable. When pentaerythritol was added stepwise and the acid formed was neutralized continuously, 95–100% yields were obtained in concentrations of the order of 60 g/l.
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