The synthesis of thermostable, strongly basic Anion-Exchange resins using Cross-Linked biguanide and its application in the extraction of Sodium copper chlorophyllin

Abstract

Commercially available, strongly basic anion-exchange resins with quaternary ammonium groups have been widely used in the purification of natural plant extracts. However, under the condition of high temperature (greater than 60 °C), these resins could not be used for long periods because of the Hofmann degradation of the strongly basic groups. In this work, the synthesis of novel, thermally stable, strongly basic resins, which has a cross-link biguanide structure, was reported. The mechanism of thermal degradation was investigated, and the result indicated that not only the stability of the functional group but also the link mode between the functional group and the resin matrix should influence the thermal stability of the resin. In our experiment, the PDG2 resin was selected to separate sodium copper chlorophyllin (SCC), a type of edible pigment derived from plants, due to its optimal thermal stability and adsorption capacity. The adsorption mechanism and thermodynamics of PDG2 were also investigated. The results demonstrated that the main adsorption affinity of PDG2 toward SCC was due to the synergistic effects of the hydrophobic and ionic interactions, and the rise in temperature will benefit the adsorption equilibrium, which differed from the equilibrium for lutein. Therefore, under suitable gradient desorption conditions, a high-purity SCC extract was prepared. After eight cycles, the adsorption capacity of the PDG2 remained constant and reproducible at a high temperature (70 °C).