Abstract
In order to explore the pathway of non-structural carbohydrate synthesis, an analysis of the pathway of non-structural carbohydrate synthesis under the network security model was proposed. Taking non-structural carbohydrates as the research object, the experimental materials and equipment were selected under the network security model. Through the establishment of detection methods, the preparation of freeze-dried carbohydrates, the influence of synthesis pathway-specific inhibitors on the synthesis of non-structural carbohydrates, the influence of precursors and intermediates in the pathway on the synthesis of non-structural carbohydrates, the effect of multiple factors on the synthesis of non-structural carbohydrates, the influence of polysaccharide synthesis, the treatment of reaction solution for detection, the preparation of detection sample, the detection conditions of a liquid phase, the detection conditions of LC-MS and the determination of carbohydrate biomass were studied. The results showed that the synthesis of nonstructural carbohydrates requires the participation of the glycolysis, shikimic acid and phenylpropane pathways, but not the polyketone pathway.
Highlights
With the successful completion of the research program of compound synthesis in the 1990s, the research on carbohydrate officially kicked off and opened a new door for human beings to explore the mysteries of life
More than 50 years ago, it was found that carbohydrates can produce polysaccharides, and only after the completion of the element group sequencing of the compounds, could the pathway of non-structural carbohydrate synthesis be fully revealed
Carbohydrate and plasmids The carbohydrates used in this paper are shown in Table 1, and the original plasmids used are shown in Organism name E. coli XL-1
Summary
With the successful completion of the research program of compound synthesis in the 1990s, the research on carbohydrate officially kicked off and opened a new door for human beings to explore the mysteries of life. In the related research of cellulose synthesis pathway, through the methods of element group location analysis and element mining, the complete functional element cluster and key regulatory factors are obtained, and the control of the whole synthesis flow is realized [4]. In laboratory analysis, many of the related elements encoding secondary synthesis in carbohydrates are silent and not expressed In this regard, the silencing element cluster can be activated by certain methods, so as to obtain more new carbohydrates with biological activity, and provide the possibility for the development and production of new products [6]. More than 50 years ago, it was found that carbohydrates can produce polysaccharides, and only after the completion of the element group sequencing of the compounds, could the pathway of non-structural carbohydrate synthesis be fully revealed.
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