The synthesis and characterization of uridine 5′-(β- l-rhamnopyranosyl diphosphate) and its role in the enzymic synthesis of rutin

Abstract

Uridine 5′-(β- l-rhamnopyranosyl diphosphate) was synthesized by the condensation of uridine 5′-diphenylpyrophosphate and β- l-rhamnopyranosyl phosphate. That sugar 1-phosphate was made via the phosphitylation of the hemiacetal hydroxyl group of 2,3,4-tetra- O-acetyl-β- l-rhamnopyranose. An enzyme preparation from the primary leaves of mung bean ( Phaseolus aureus) was shown to catalyze the transfer of l-rhamnose from UDP-β- l-rhamnose to the flavonol d-glucoside isoquercitrin to form rutin.