The Synergistic Effect of Ozonated Oil and Desensitising Toothpaste on Dentinal Tubule Occlusion: An In Vitro Study.

Abstract

Introduction Dentin hypersensitivity (DH) is the most common problem encountered by clinicians. It can bemanaged either by blocking neural activities or by occluding tubules. Ozonated oil facilitates the simple passage ofdesensitizing agents into dentinal tubules. Aim This study aimed to evaluate the effect of ozonated oil on dentinal tubule occlusion before and after the application of desensitizing toothpaste. Materials and methods The study was carried out in Kalinga Institute of Dental Sciences, KIIT (Deemed to be University), Bhubaneswar, India.The sample size of the study was 80. The first group contained specimens for control. The second group comprised specimens treated with ozonated oil. The third group comprised specimens being treated with a desensitizing agent. The fourth group contained specimens treated with both the desensitizing agent and ozonated oil. The specimens then received an acid challenge. The specimens were observedunder a scanning electron microscope (SEM) before any therapy, after the application of the therapeutic agents and after the 37.5% ortho-phosphoric acid challenge. Results Inferential statistics to compare between the groups was calculated using one-way analysis of variance (ANOVA) statistics. Post-hoc Tukey'shonestly significant difference (HSD) was performed to compare the groups. The mean scoresof the partial tubular occlusion of Group 1, Group 2, Group 3and Group 4 before the acid challenge were0.035, 0.691, 0.564and 0.368, respectively. The maximum score was obtained in the case of Group 2, which was statistically significant. The mean scores for partialtubule occlusionafter the acid challengeforGroup 1, Group 2, Group 3and Group 4 were0.055, 0.531, 0.733and 0.142, respectively. There was evidence of maximumpartialtubule occlusion after the acid treatment in the case of Group 3. The mean scores of Group 1, Group 2, Group 3and Group 4 before the acid challenge were 0.019, 0.309, 0.442 and 0.609, respectively. The maximum score was obtained in the case of Group 4, implying a greater number of total tubular occlusions before the acid challenge.The mean scores of the total tubular occlusions after the acid challenge for Group 1, Group 2, Group 3and Group 4 were0.047, 0.465, 0.272 and 0.890, respectively. There was evidence of maximum tubule occlusion in the case of Group 4, which was statistically significant. Conclusion Overall, the application of a desensitizing toothpaste with ozonated oil holds promise as a potentially more effective treatment approach for DH. Further research and clinical studies may be needed to fully validate its efficacy and safety in dental practice.