The synergic effects of CTLA-4/Foxp3-related genotypes and chromosomal aberrations on the risk of recurrent spontaneous abortion among a Chinese Han population

Qin’E Fan,Chaoyun Wang,Yu Cui,Juanjuan Zhang,Yongjun Xie,Libing Wu,Qiurong Wang

doi:10.1038/s10038-018-0414-2

Qin’E Fan, Chaoyun Wang + Show 5 more

Open Access

https://doi.org/10.1038/s10038-018-0414-2

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Abstract

The current study was aimed to investigate the association of CLTA-4/Foxp3 polymorphisms and chromosomal abnormalities with recurrent spontaneous abortion (RSA) risk in a Chinese Han population. Altogether, 1284 RSA women and 1046 women with normal pregnancy were incorporated in this study. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was implemented to genotype the single-nucleotide polymorphisms (SNPs) located within CTLA4 and Foxp3. Moreover, the cytogenetic diagnosis was performed in line with the standards of G banding karyotype. As a consequence, rs231775 and rs3087243 of CTLA4, as well as rs2232365 and rs2232368 of Foxp3, all appeared to modify the risk of RSA. Besides, significant differences were found between the ratio of structural abnormality and that of numerical abnormality (P < 0.038), and chromosome abnormality was associated with higher miscarriage frequency (>3) than normal karyotypes. Of note, the synergic effects of the genotypes and chromosomal abnormality all tallied with the sub-multiplication model (ORchromosome × ORSNP > ORchromosome+SNP), while rs2232365 GG and chromosomal aberration impacted the RSA risk in a super-multiplicative way that ORchromosome × ORSNP < ORchromosome+SNP. In conclusion, susceptibility to RSA was subject to the synthetic regulation of chromosomal aberrations and genetic mutations within CLTA-4 and Foxp3, suggesting that the conduction of karyotype analysis and genetic detection for RSA patients could effectively guide effective RSA counseling and sound child rearing.

Highlights

Recurrent spontaneous abortion (RSA) is referred to that abortions are continuously repeated for ≥2 times [1]
Aberration of single-nucleotide polymorphisms (SNPs) that were located in the promoter region of Foxp3, such as rs2232365 and rs3761548, could directly bring about frame shift mutations, and thereby deranging the functions of Foxp3 transcript [5]
CTLA-4 combined with B7 not merely inhibited the multiplication and activation of T cells, and held up the cell phrase within G0/G2 stages, so that T cells were limited by inability of proliferating and secreting cytokines [29]

Summary

Introduction

Recurrent spontaneous abortion (RSA) is referred to that abortions are continuously repeated for ≥2 times [1]. Aberration of single-nucleotide polymorphisms (SNPs) that were located in the promoter region of Foxp, such as rs2232365 and rs3761548, could directly bring about frame shift mutations, and thereby deranging the functions of Foxp transcript [5]. Among a great diversity of SNPs that affected the CTLA4 actions [13,14,15,16], it was demonstrated that the mutated genotypes of rs231775 and rs3087243 not merely contributed to depressed level of serum CTLA4, and created larger risk of RSA [9, 17, 18]. Diverse studies have been concentrated on the role of CTLA-4/Foxp SNPs or chromosomal polymorphisms in regulating the RSA risk, few studies were aimed to study their synthetic effects. The current study was purposed to remedy this gap, which might provide evidences for finding novel treatment strategies for RSA

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