The Synbiotic Healing Effect of Probiotics and Prebiotics on Indomethacin Induced Gastric Ulcer in Rats

Abstract

Background : Probiotics are becoming one of the main alternative medicational solutions used worldwide nowadays. Besides to their function in alleviating the immune system, they are used for the treatment of gastric ulcer. Along with the mixing with prebiotics, this could increase the healing percentage and maybe rate as well. Methodology : In this study, the synbiotic effect of the Bifidobacteria and lactobacillus probiots with the Punica granatum, Glycyrrhiza glabra, Cynara cardunculus. Prebiots were experimented separately to identify whether they would increase the healing effect or not. We divided the Ulcer-induced rats into 13 groups and treated for 14 days. Results: The results showed a decrease in WBCs count in the synbiotic mixture of Lactobacillus and P. granatum followed by Bifidobacterium with P. granatum. The lactobacillus and Cynara cardunculus mixture gave the highest WBCs count. Also, a decrease in both IL6 and IFN-γ after treatment with all synbiotic mixtures. The histopathology showed decreased gastric lesions and infiltration by mononuclear cells in all mixtures where the lactobacillus, P. Granatum mixture showed the best healing effect compared to the rest of groups and Ranitidine reference group. These results suggest that using probiotics along with prebiotics could be a potential treatment of gastric ulcer via enhancing the immune system.