Abstract
When subterranean clover (Trifolium subterraneum L.) plants were grown for 3 weeks over distilled water, tryptophan could be detected in the root medium of both sterile cultures and those inoculated with Rhizobium trifolii 3 days earlier. Auxin could be detected only in the inoculated medium. The auxin had the chromatographic and growth properties of indole�3-acetic acid (IAA). Since nodule bacteria produce auxin only in the presence of tryptophan, which is a probable precursor of lAA, it is suggested that the tryptophan exuded by clover roots is converted to lAA by nodule bacteria. Auxin was still produced in the root medium when strains of Rhizobium which do not nodulate subterranean olover roots were used as inooulant, or when nitrate, which delays nodulation, was present in the medium.
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