Abstract
Osteogenic potential of the HOS cells, a cell line derived from the human osteosarcoma, and KHOS cells, HOS cells transformed by the Kirsten murine sarcoma virus, was studied biochemically and histochemically by analyzing the expression of two osteogenic differentiation markers of the bone cells. Both the expression of the liver/bone/kidney-type ALPase and deposition of calcium were observed in the HOS cells, while the expression of both differentiation markers were suppressed in the KHOS cells.Southern and Northern blot analyses suggested that in the KHOS cells a single copy of the intact K-MSV proviral DNA was integrated and the 6.5 kb K-MSV RNA was transcribed from the integrated DNA. The expression of the K-MSV genome in the KHOS cells is discussed in relation to the suppression of the expression of osteogenic differentiation markers.
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