Abstract
The NKX2.1 gene is considered as a proto-onco gene to promote the development of early lung adenocarcinoma. In this study, three kinds of specific siRNAs aiming to NKX2.1 gene were designed to down-regulate the expression of NKX2.1 gene, and constructed into rAAV vectors, respectively. NCI-H1975 cells were infected with the rAAV virus. RT-PCR results showed that all the three siRNAs decreased the expression of NKX2.1 mRNA. WB results show that the expression of NKX2.1 protein was decreased too. It is concluded that the siRNA constructed in rAAV could significantly down-regulate the expression of NKX2.1 gene. Meanwhile, it is proven that the rAAV-NKX2.1-siRNA could inhibit the proliferation and induce apoptosis occurrence in NCI-H1975 cells. In this study, we have expected that the rAAV-NKX2.1-siRNA could be developed into a potential genetic engineering therapeutic virus to suppress the lung AC development and metastasis. The current experiment thus makes a foundation for further research on in vivo gene therapy.
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