The Sugar Transporter family in wheat (Triticum aestivum. L): genome-wide identification, classification, and expression profiling during stress in seedlings.

Hongzhan Liu,Lili Li,Zhongke Sun,Guihong Yin,Guangyu Yang,Keshi Ma,Lizong Hu,Xianle Ruan,Xueqin Wang,Junsheng Wang,Lin Qiao,Chaoqiong Li,Chunping Wang

doi:10.7717/peerj.11371

Abstract

The sugar transporter protein (STP) plays a crucial role in regulating plant growth and stress tolerance. We performed genome-wide identification and expression analysis of the STP gene family to investigate the STPSs’ potential roles in the growth of wheat seedlings under stress. Here, a total of 81 TaSTP genes containing the Sugar_tr conserved motif were identified within the wheat genome. Bioinformatic studies including phylogenetic tree, chromosome position, and tandem repeat were performed to analyze the identified genes. The 81 TaSTP genes can be classified into five main groups according to their structural and phylogenetic features, with several subgroups, which were located separately on chromosomes A, B, and D. Moreover, six gene clusters were formed with more than three genes each. The results of three comparative syntenic maps of wheat associated with three representative species suggested that STP genes have strong relationships in monocots. qRT-PCR analysis confirmed that most TaSTP genes displayed different expression profiles after seedlings were subjected to six days of different stress (10% PEG6000, 150 mM NaCl, and their combination, respectively), suggesting that these genes may be involved in regulating plant growth and stress tolerance. In conclusion, 81 TaSTP genes were identified and their expressions changed under stress, indicating TaSTP’s potential roles in wheat growth monosaccharide distribution is regulated.

Highlights

Sugar is used as a universal energy source in higher land plants, playing important roles in cell development, signal transmission, and osmotic homeostasis under certainHow to cite this article Liu H, Li C, Qiao L, Hu L, Wang X, Wang J, Ruan X, Yang G, Yin G, Wang C, Sun Z, Ma K, Li L. 2021
In the process of apoplastic unloading, invertase catalyzes the irreversible hydrolysis of sucrose into glucose and fructose, which is unloaded by monosaccharide transporters (MST)
A total of 390 putative TaSTP genes were identified in the wheat genome through a HMMER analysis

Summary

Introduction

Sugar is used as a universal energy source in higher land plants, playing important roles in cell development, signal transmission, and osmotic homeostasis under certainHow to cite this article Liu H, Li C, Qiao L, Hu L, Wang X, Wang J, Ruan X, Yang G, Yin G, Wang C, Sun Z, Ma K, Li L. 2021. Sucrose is the main form of long-distance carbohydrate transport in higher plants, while SUT, invertase, and MST are important in the loading and unloading of sugar molecules (Slewinski, Meeley & Braun, 2009; Zhang, Zhang & Liu, 2015; Doidy, Vidal & Lemoine, 2019). Almost all STPs are a high affinity hexose transporter with specific expression in tissues, indicating that one of its main functions is to build up the sink required for photosynthate redistribution (Büttner, 2007; Schofield et al, 2009) This process plays a key role in maintaining source/sink characteristics and hormonal signals, as highlighted in the case of abiotic or biotic stresses (Balibrea Lara et al, 2004; Roitsch & González, 2004)

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Conclusion