Abstract
Sucrose synthase (SS) is a key enzyme involved in sucrose metabolism that is critical in plant growth and development, and particularly quality of the fruit. Sucrose synthase gene families have been identified and characterized in plants various plants such as tobacco, grape, rice, and Arabidopsis. However, there is still lack of detailed information about sucrose synthase gene in pear. In the present study, we performed a systematic analysis of the pear (Pyrus bretschneideri Rehd.) genome and reported 30 sucrose synthase genes. Subsequently, gene structure, phylogenetic relationship, chromosomal localization, gene duplications, promoter regions, collinearity, RNA-Seq data and qRT-PCR were conducted on these sucrose synthase genes. The transcript analysis revealed that 10 PbSSs genes (30%) were especially expressed in pear fruit development. Additionally, qRT-PCR analysis verified the RNA-seq data and shown that PbSS30, PbSS24, and PbSS15 have a potential role in the pear fruit development stages. This study provides important insights into the evolution of sucrose synthase gene family in pear and will provide assistance for further investigation of sucrose synthase genes functions in the process of fruit development, fruit quality and resistance to environmental stresses.
Highlights
In higher plants, sucrose is an essential element of the life cycle
In the third category, we identified a series of stresses-related elements, such as HSE involved in heat stress, ARE for anaerobic induction, Box-W1 for fungal elicitors, GC-motif for anoxia, and TC-rich repeats involved in stress responses
The sucrose synthase gene family has been identified in various plants, such as citrus
Summary
Sucrose is an essential element of the life cycle. It is mainly produced by photosynthesis source tissues and transported to sink tissues where it serves as a carbon and energy source for the various metabolic pathways. The utilization of sucrose in plant cells requires its cleavage, which is performed by two key enzymes, sucrose synthase (UDP-glucose: D-fructose-2-glucosyltransferase, EC 2.4.1.13, EC 2.4.1.13) and sucrose cleavage invertase. Sus contributes to dispensing of carbon resources into several pathways that are essential for the metabolic and storage physiology of plant cell [4,5,6]
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