Abstract

Analysis of brush-border and basolateral membrane prepared from the epithelial cells of rat small intestine showed that the brush border contained less of some phospholipids and more glycosphingolipids per protein. The N- glycoloylneuraminosyllactosylceramide was enriched in the brush border, while cholesteryl sulphate and fucosyllactosylceramide were more prominent in the basolateral membrane. By periodate-NaB 3H 4 labelling of the intact small intestine, it was shown that N- glycoloylneuraminosyllactosylceramide is exposed at the luminal cell surface of the epithelial cell.

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