The Study On The Relationship Between c-Myc and Hif-1 α In u266 Cell Line

Abstract

Objective The aim of study is to explore the relationship of C-MYC and HIF-1 α in multiple myeloma cell lines U266,and research the influence of C-MYC/HIF-1 α on VEGF, in order to find a new target for clinical treatment MM. Methods Transfect the siRNA c-myc plasimd,siRNA HIF-1 α plasimd and mock plasimd into U266 cell lines respectively.RT-PCR was used to detected the expression of C-MYC and HIF-1αin different group, (fetch the CT value, using 2-ΔΔCT to calculate) .The expression of protein of C-MYC.HIF-1αand VEGF was measured with Western-blot in different group,((using the ratio of purpose gene and internal reference β-actin expression as the final results). Results compared with the control group, the expression of C-MYC, HIF-1 α and VEGF was decreased in the siRNA c-myc group, which differences are statistically significant (p<0.05), the expression of HIF-1 α and VEGF was decreased in the siRNA c-myc group ,which differences are statistically significant (p<0.05),the expression of C-MYC have no obvious decline compared with control group. Conclusion The expression of HIF-1 α depends on the regulation of C-MYC gene, synthesis and secretion of VEGF depends on C-MYC/HIF-1 α, C-MYC/HIF-1 α may serve as a new target for treatment of MM. Disclosures: No relevant conflicts of interest to declare.