The study on the effects of apigenin on the proliferation in human androgen-independent prostate cancer cell PC-3 and the role Caspase-3 involved in

Abstract

Objective To observe the effect of apigenin (API) on the proliferation of human androgen-independent prostate cancer cell line PC-3 and molecular mechanisms.Methods Clutures of PC-3 cell were exposured to API at the different concentrationa of 0,10,20 and 40 μmol/L respectively.The effect of API on growth inhibition were determinated with morphometry,cell counting kit-8 (CCK-8) assay.The expression of Caspase-3 mRNA was determined by reverse transcriptase-polymerase chain reaction (RT-PCR).Results CCK-8 assay showed that the absorbance were 1.48 ±0.04,0.81 ±0.04,0.69 ± 0.03 and 0.33 ± 0.03 respectively when 0,10,20,40 μmol/L API treated PC-3 cells for 48 h,compared to the control group,10,20,40 μmol/L groups significantly inhibited the proliferation on PC-3 cells,the inhibition rates were 44.7％,53.5％,77.4％ (P ＜ 0.01).The Caspase-3 mRNA expression of 0,10,20,40 μmol/L group were 0.230 ± 0.036,0.410 ± 0.080,0.480 ± 0.100,0.730 ± 0.150,there was statistically significant differences between experimental and control groups (P ＜ 0.01).Conclusion The growth inhibition could be taken by API in PC-3 in a dose-dependent manner.The mechanism was that high expression level of Caspase-3 was caused by API,which led to the apoptosis of PC-3. Key words: Apigenin; Androgen-independent prostate cancer; Apoptosis; Caspase-3