The study on effect of the expression of interleukin-35 in HepG2 cell by lipopolysaccharide induced

Abstract

Objective To investigate the effects of lipopolysaccharide (LPS) on the expression of p35 and Ebi3 subunits in HepG2 cell and the effects of LPS on proliferation and apoptosis of HepG2 cell line in vitro. Methods The effects of LPS on the cell proliferation activity of HepG2 in vitro was measured using cell counting kit-8 (CCK-8) assay and cell apoptosis was detected by Flowcytometry (FCM). FCM were used to test the expression level of p35 and Ebi3 in the LPS treated groups and control group. Enzyme-linked immunosorbent assay (ELISA) were used to test the cultured supernatants expression levelof IL-35. Results The cell proliferative activity were 0.66±0.07, 0.67±0.05, 0.68±0.09 after stimulation with LPS group (10, 100, 1000 μg/L) for 24 hours. Compared with the control group were statistically significant (P values were 0.031, 0.017, 0.007 respectively). The content of IL-35 protein in LPS (100 ng/ml) treated groups [(77.06±35.15) ng/L] had significantly higher compared with control group [(34.13±19.95) ng/L, P=0.035]. The content of p35 and Ebi3 had significantly higher when compared with control group (P values were 0.011 and 0.019 respectively). Conclusion LPS can promote proliferation of HepG2 cells and regulate the expression of interleukin-35 in HepG2 cells. Key words: Lipopolysaccharide; HepG2 cell; Proliferation