Abstract
In our research, we explored the relationship between Keratin 26 and the regulation of fine hair, BMP signaling pathway, MT, FGF5, and IGF-I. The result of hybridization in situ revealed that Keratin 26 was specially expressed in cortex of skin hair follicles; the result of immunohistochemistry indicated that Keratin 26 was expressed in internal root sheath, external root sheath. Then, Real-time quantitative PCR results showed that relative expressive quantity of Keratin 26 was 1.08 or 3.3 × greater in secondary follicle than primary follicle during anagen or catagen; the difference during anagen was not remarkable (p>0.05), however, that of catagen was extremely significant (p<0.01). Relative expressive quantity of Keratin 26 increased during telogen; the difference was extremely significant (p<0.01). Moreover, after Noggin expression interference using RNAi technology, we found that relative expressive quantity of Keratin 26 extremely remarkably declined (p<0.01); after K26 overexpression, we found that relative expressive quantity of Noggin extremely remarkably increased (p<0.01). We detected expressive quantity change of Keratin 26 and Keratin 26 using Real-time quantitative PCR and immunofluorescence technologies after fibroblasts were treated with MT, FGF5 or IGF-I; the results indicated that MT and FGF5 played a positive role in Keratin 26 and Keratin 26 expression, IGF-I played a negative role in Keratin 26 expression, positive role in Keratin 26 expression. The results above showed that Keratin 26 could inhibit cashmere growth, and was related to entering to catagen and telogen of hair follicles; Keratin 26 and BMP signaling pathway were two antagonistic pathways each other which could inhibit growth and development of cashmere; MT, FGF5 and IGF-I could affect expression of Keratin 26 and Keratin 26, and Keratin 26 was one of the important pathways that MT induced cashmere production in advance, FGF5 regulated cashmere growth and IGF-I promoted cashmere growth and development.
Highlights
Goat cashmere grows in outer surface cortex of goat skin
We found that many keratins in human and sheep all were expressed in skin hair follicles and closely related to hair growth, bone morphogenetic proteins (BMPs) signaling pathway was closely associated with hair follicle development and cashmere growth, those indicated there was some relationship between keratins and BMP signaling pathway, we need to further study and confirm it
Quantitative real-time polymerase chain reaction (PCR) experiment results showed that keratin 26 relative expression quantity was 1.08 × and 3.3 × greater in secondary follicles than primary follicles during anagen and catagen (Fig 5A and 5B), the difference of anagen was not significant (p > 0.05), but the difference of catagen was extremely significant (p < 0.01); because primary and secondary follicles couldn’t be distinguished clearly during telogen of hair follicles, we detected keratin 26 expression of skin, the results indicated that keratin 26 relative expression quantity during this period was higher than that of anagen and catagen periods (Fig 5C), the difference was all extremely significant (p < 0.01)
Summary
Goat cashmere grows in outer surface cortex of goat skin. Goat cashmer is very percious, its yield is rare and quality is excellent. Seventy percent of cashmere all over the world comes. The Study on Biological Function of Keratin 26 from Liaoning Cashmere Goat. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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