The study of volume density of tracheal ganglions in vitro in new born babies with respiratory distress syndrome.

Ragip Shabani,Ilir Kurtishi,Naim Haliti,Ruke Beqiri,Hilmi Islami,Ramadan Dacaj,Fehmi Zeqiri,Sadi Bexheti,Labinot Shahini

doi:10.17305/bjbms.2009.2790

Ragip Shabani, Ilir Kurtishi + Show 7 more

Open Access

https://doi.org/10.17305/bjbms.2009.2790

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Abstract

Volume density of respiratory organs was studied in vitro in newborn babies at different age of gestation (abort, immature, premature and mature) using stereometric method. The total of 23 cases was subject to this study. The respiratory organs (trachea, lungs) were taken from autopsies of newborn babies exited from different causes. For this purpose the tissues were fixed in formalin (10%) solution, cut serially in 7micro and 10micro slabs. Volume density of the respiratory system was assessed stereometricaly using Universal testing system Weibel M 42. We observed that volume density of epithelia, musculature and glands were proportionally present in the tracheal tissue. Cellular interstitial tissue is consistently increasing and corresponds to the developmental stages of the newborn babies. The density of tracheal ganglions is greater in premature ages of immature and premature newborns (p<0,05). Decreased number of ganglion cells is observed in mature ages (p<0,05). This is caused by intensive ramification of ganglions from serosa to deeper layers of trachea right to epithelium. Medium diameter of tracheal ganglions is greater in mature newborn babies and corresponds to developmental ages of babies.

Highlights

The structure of ganglions found in the lungs is very complex; these structures are studied while making intracellular measurements in human material ( )
Stereometric study has been made in serial sections of tracheal tissue, where presented density and medium diameter of ganglions, epithelium, glands, smooth musculature, cartilage and interstitial was given in volume units
The study shows that sympathetic nerve fibers of the trachea stem from superior cervical ganglion and stellate ganglion

Summary

Introduction

The structure of ganglions found in the lungs is very complex; these structures are studied while making intracellular measurements in human material ( ). Neurophysiological studies of ganglions show at least - groups of neurons. Ganglions integrate sensory impulses for local control of smooth musculature. Studies in humans have shown that intramural plexus of trachea and bronchia is formed by the end of fetal period. Parasympathetic bronchial innervation in developed phase, shows that paraganglionar vagus nerves, which innervate smooth musculature of respiratory airways, are involved in breathing control before birth ( ). Tracheal ganglions are parasympathetic structures, and contain sympathetic sensory fibers. These problems are explained by several immunohystochemical methods ( ). Noradrenergic nerve fibers are found around blood vessels in mucosa and smooth musculature of trachea, but are never observed near ganglion neurons ( )

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