The study of virus collation with the polymerase chain reaction (PCR) method in export fishery commodities

Abstract

Fish trade traffic from within and outside the country leads to the development of changes in pests and fish diseases in Indonesia. One of the infectious diseases known to attack the Indonesian exports of fishery commodities is a virus and can be identified using Polymerase Chain Reaction (PCR). The goal is to know the types of fish and shrimp and their export commodities with the examined samples virus and virus checking procedures with PCR. The method was used descriptive method. Stages of PCR consist of extraction of DNA/RNA, amplification using the kit IQ2000 ™ and Access Quick RT-PCR System, electrophoresis, and diagnostic test results on Uvidoc. Types of fish and shrimp samples for examination were freshwater fish, lobster, broodstock vanamei, red lobster, fries, and seawater ornamental shrimp—the type of virus that would be examined in accordance with the provision of export destinations. During street vendors’ activities, total samples of the fish and shrimp virus examination of export commodities were 39 samples declared negative, whereas two samples declared positive were WSSV.