The study of toxic effects of toxic isolate Alternaria alternata in vivo of white mice and the ability of Biological and Chemical treatments in the reduction of toxicity

Abstract

The study included the detection of the effect of the Mycotoxins of the fungal isolates of Alternaria alternata, which was diagnosed both genetically and chemically by PCR. The analysis showed that the ability of A. alternata to produce toxins, including AltratoxinII, was found in one of the isolates 7.5499μg / ml. This concentration is used in the experiment to determine its effect on the tissues and organs of white mice. In the study, the fungus Pleurotus ostreatus was used to reduce the toxicity of the fungus A. alternata. The fungus P.ostreatus gave high tolerance to A. alternata and evaluated the effect of sequential chemical treatments CaCO3 on reducing the auditory effect on laboratory mice. The toxic isolation of pathogenic fungi is extract into the body of the organism. The effect on the levels of liver enzymes (GPT, GOT, ALP) has reached its level (IU / L) (84, 88, 264) respectively compared to control group which reached (22, 35, 147) IU / L respectively as well, And resulted in the effectiveness of the decomposition of liver cells containing enzymes and led to the liberation of the serum, as well as the cause of changes in the tissue pathological clear in the cross section of the liver tissue, including the occurrence of hepatocytes vaculosis and the presence of bubbles in the cells walls and the expansion of liver sinuses causing Hydropic degeneration. The results of the laboratory examination of the kidney function showed significant differences in the increase in the concentration of urea and creatinine in the serum of laboratory mice treated with urea level (59 mg / dl) and creatinine (1.73 mg / dl) compared to control group (29 mg / dl) And (0.7 mg / dl) respectively, and found a clear effect within the kidney tissues leading to atrophy in the glomeruli, infiltration of the lymphocytes and hemorrhage, in addition to the degradation of urinary tubule cells affecting kidney function leading to renal failure.