The study of the protective properties of vitamin E on bone marrow of rats in condition of the increased intake of heavy metals salts

Abstract

Homeostasis imbalance leads to the changes in qualitative and quantitative characteristics of all hematopoietic sprouts. It encourages to search for the effective protective means in the conditions of the polluted environment. Our research is focused on the study the protective properties of vitamin E on the bone marrow of the rats in conditions of the increased intake of the heavy metals salts. During the research the bone marrow of the 36 laboratory male rats was studied. The sections of the decalcified femoral bones were stained using hematoxylin and eosin method, Van Gieson’s technique and PAS-reaction, as well as immunohistochemical study to CD3, CD79α, S100 and MРО was used. Elemental composition of hematopoietic tissue was determined through atomic spectrophotometry. The accumulation of the heavy metals led to microelementosis (changes in qualitative and quantitative characteristics of all hematopoietic sprouts and surrounding tissues). The extension of the influence of the exogenous pollutants led to the suppression of erythro-and leucopoiesis, synchronous increase in the number of megakaryocytes. These indicators reached the significant deviations on the 90 day of the observation. The use of the vitamin E as a corrector reduces the negative effect of the pollutants that is demonstrated by the reduced accumulation of the heavy metals in the bone marrow, prevention of the significant deviations in the quantitative and qualitative composition of hematopoietic tissue.