Abstract
The protection of neurons from damage and death is an important challenge in the development of treatment of brain ischemia and neurodegenerative diseases. This study aims to investigate protective effect of the extract prepared from Chelidonium majus, which contains total alkaloids. In the present study, we examined antioxidant activity of total alkaloids from Chelidonium majus in vitro. Hydroxyl radical and lipid radicals were detected using spin trapping agents with ESR spectrometer. Chelidonium majus extract exhibited dose-dependent scavenging effects on lipid radicals. Halfmaximal inhibitory concentration (IC50) of the extract was 2.96 mg/ml, whereas for hydroxyl radicals it was 55.13 mg/ ml. These results showed that extract of Chelidonium majus is partly inhibited free radicals. Antioxidant effects of this extract were further demonstrated by protecting enzyme activity of the mitochondrial respiratory electron transport chain (complex I) in isolated brain mitochondrial dysfunction induced by MDA. However, it did not change the decreased level of complex II, and malate dehydrogenase (MDH) in a concentration of 12 and 25 mg/ml. Here, we examined the neuroprotective effect of the extract from Chelidonium majus against oxidative stress in cultured cortical neurons, using MTT. We found that pretreatment with the extract of Chelidonium majus (0.05 and 0.5 mg/ml) significantly inhibited H2O2-induced cell death in cortical neurons.Furthermore, the use of a luciferase reporter (ARE-luc) assay showed that extract from Chelidonium majus activates protective signaling pathway in primary cortical neurons through ARE/Nrf2 pathway.Together, this suggests that total alkaloids from Chelidonium majus may be neuroprotective by increasing anti-oxidant gene expression.
Highlights
Чистотел большой Chelidonium majus L. – многолетнее травянистое растение, принадлежащее семейству маковых (Papaveraceae)
We found that pretreatment with the extract of Chelidonium majus (0.05 and 0.5 mg/ml) significantly inhibited H2O2-induced cell death in cortical neurons
Itoh K, Wakabayashi N, KatohY, Ishii T, Igarashi K, Engel JD, et al Keap represses nuclear activation of antioxidant responsive elements by Nrf through binding to the amino-terminal Neh domain, Genes Dev. 1999; 13(1), 76-86
Summary
Изучение нейропротективного действия экстракта чистотела большого (Chelidonium majus L.) in vitro. Резюме Целью данного исследования было изучение нейропротекторного действия экстракта чистотела большого (Chelidonium majus L.). Экстракт чистотела большого продемонстрировал значительное ингибирование свободных липидных радикалов в ESR-анализе, но не уменьшал образование гидроксильных радикалов. Также оценено защитное действие экстракта чистотела большого против митохондриальной дисфункции, вызванной 100 мкМ малондиальдегида, в изолированных митохондриях клеток головного мозга. Экстракт чистотела большого в концентрациях 12 и 25 мг/мл достоверно увеличивал пониженный уровень комплекса I, что указывает на проявление его антиоксидантного действия. Также мы изучили защитное действие экстракта против нейротоксичности, вызванной перекисью водорода (H2O2) в культивированных кортикальных нейронах. Что экстракт чистотела большого в концентрациях от 0,05 до 0,5 мг/мл достоверно снижает гибель нейронов, вызванную токсическим действием перекиси водорода (100 мкМ, H2O2). The Study of the Neuroprotective Effect of the Extract from Chelidonium Majus L. in Vitro
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