The study of the consequences of preserving berry crops in vitro on the processes of subsequent clonal micropropagation

Inna Knyazeva

doi:10.1051/bioconf/20213005004

Abstract

As a result of studies, it was found that collection samples of berry crops in vitro can be maintained in viable condition on specially selected nutrient media for 12 months. The effect of mannitol concentration (0.45, 0.75 and 1.05%; sucrose control -3%) on the regenerative potential of microprobe of the studied berry crop varieties was estimated. When studying the effect of the reproduction cycle on the morphogenesis of meristemic cultures, it was found that the dynamics of changes in shoot length, number of leaves and shoots depended on the concentration of the active substance. In general, the majority of berry crops that were viable after storage of explants retained the ability to grow again, in many cases reaching or exceeding the control level.

Highlights

Plant genetic resources serve as a strategic basis for the effective stable development of agriculture, and of all sectors of the economy and social sphere of the Russian Federation and the world as a whole [1,2,3]
Shoots of approximately 1.5 cm long were used, which were placed in test tubes (150x160 mm) pre-sterilized with dry heat (150-200°C) with 10 ml of agarized Murashige and Skoog nutrient medium (MS) supplemented with mannitol in concentrations 0.45% (A), 0.75% (B), 1.05% (C), sucrose control 3% and 0.7 mg L-1 6-benzylaminopurine (BAP), 0.1 mg L-1 indole-3-butyric acid (IBA) and 8 g L1 agar-agar (Panreac, Spain), pH 5.7
As a result of studies it was found that collection samples of berry crops in vitro can be maintained in a viable state for 12 months on specially selected nutrient media. (Fig. 1)

Summary

Introduction

Plant genetic resources serve as a strategic basis for the effective stable development of agriculture, and of all sectors of the economy and social sphere of the Russian Federation and the world as a whole [1,2,3]. The multiplication rate of strawberry and raspberry shoots in the first subculture after storage was lower than that of non-stored cultures. Berry crop regeneration protocols are available for different varieties, there is limited information on individual varietal and hybrid profiles in the direction of restoring explant viability after direct cultivation in vitro. The purpose of these studies was to establish the patterns of the aftereffect of medium-term conservation of in vitro berry culture explants on the processes of subsequent clonal micropropagation

Experimental

Results and discussion

Conclusion

Gene bank standards for plant genetic resources for food and agriculture