Abstract

Due to the unique properties, nanoparticles (NPs) have been widely used both in industrial products and individual life. However, with the huge benefits, NPs also possess potential toxicity to human health and ecosystem. Aluminum oxide NPs is so significant in industry that it occupies the second important position in US nanomaterials market. Aluminum oxide NPs belong to metal oxide nanoparticles which may have strong antimicrobial properties. As there is limited prior research on the toxicity of alumina NPs, the main goal of present research is to study the effects of alumina NPs on microorganisms in soils by combination methods of microcalorimetry and urease. Power-time curves and thermo-kinetic parameters (such as total heat evolution QT, growth rate constant k and maximum power output Pmax) were applied to evaluate the microbial community activities of the soil samples. A range of concentration of Aluminum oxide NPs gradient from 0 mg/L to 1000 mg/L was applied. The results showed that the concentration under 500 mg/L has negligible inhibitory effects on soil microorganisms while concentrations at 500mg/L and 1000 mg/L only have mild inhibition on microbes in soil. Urease experiments verified this result, therefore, it suggests that combination of microcalorimetry and urease may provide practical method for microorganism activity evaluating.

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