The study of survivin-shRNA inhibits growth of human colon cancer SW480 cells

Abstract

Objective To observe the inhibitory effect of survivin gene expression and cell proliferation of the human colon cancer cell line SW480 caused by surviving-shRNA. Methods The survivin-shRNA was constructed and transfected into SW480 cells with LipofectamineTM2000. The down-regulations of survivin expressions were detected by RT-PCR and western blot analysis. The cell proliferation inhibitions were determined by MTT assay. Results The survivin-shRNA successfully and efficiently suppressed the expression of survivin mRNA and protein(P＜0.05).The expression inhibition rates were 41.49% and 40.82% at the mRNA and protein level respectively. The MTT assay indicated that the specific shRNA resulted in significant inhibition of cell growth on the culture plates on a time-dependent manner since 48 hours post-transfection. The cell viability of SUR group was 47.8% at 72 h,which was of statistical significance compared with that of blank control group (P＜0.05).Conclusions The specific survivin-shRNA could down-regulate the expression of survivin gene and inhibit the growth of SW480 cells. Key words: Survivin ; Colon cancer ; RNA interference ; ShRNA ;