Abstract
All bacteria can survive and adapt to different stresses, such as fluctuations in temperature, pH oxidative, and osmotic pressure occurring in their surrounding environments. This study aims to evaluate the effects of a variety of stress conditions on the growth, and proteome ofRaoultella planticolaPTCC 1598.R. planticolacells were exposed to different values of temperatures, sodium chloride, pH, and hydrogen peroxide stresses. Among the stress conditions, oxidative stress, upon exposure to hydrogen peroxide (H2O2) at 4000ppm concentration was selected for proteomics analysis in detail. Approximately, 1400 spots were identified in two-dimensional gel electrophoresis (2-DE). Among the identified spots, 85 spots were repeatable using 2D-Platinum software and eye confirmation and, nine protein spots were differentially expressed. Among nine proteins, six proteins identified successfully with an MASCOT score greater than 40 (p < 0.05) were 2,3-dihydroxybenzoate-2,3-dehydrogenase (oxidoreductase family), hypothetical protein G787-04832, periplasmic D-galactose-binding protein, uridine phosphorylase (glycosyltransferases), a single peptide match to cysteine-binding periplasmic protein, and NADP(H) nitroreductase. All identified proteins showed decreased level expression. Based on the obtained results, we concluded that hydrogen peroxide as an antiseptic compound could affect cell growth and proteomics ofR. planticola. Therefore, we recommend using an antiseptic solution containing H2O2 to prevent the spread ofR. planticola as a newemerging pathogen.
Highlights
Raoultella planticola is a gram-negative, non-motile, aerobic bacillus that was first introduced by Bagley et al 1981(Bagley et al, 1981)
As shown, increasing the severity of all stress conditions reduced viable counts (CFU/ml) of R. planticola cells compared to the non -stress conditions
Shifting temperature from 37 °C to 45 °C decreased logarithm colony forming unit per milliliter (CFU/ml) of R. planticola about 1.39 fold, no bacterial growth was observed above 85°C
Summary
Raoultella planticola is a gram-negative, non-motile, aerobic bacillus that was first introduced by Bagley et al 1981(Bagley et al, 1981). R.planticula previously classified as Klebsiella planticula and Klebsiella trevisanii, first was combined into one species, i.e., K.planticula, based on DNA-DNA homology in 1986 R. planticolahas been considered as a nonclinical, aquatic, botanical, and soil organism Nowadays it is considered as an emerging pathogen that causes some cases of human infections, including bacteremia, soft tissue infection(O'Connell et al, 2010), pancreatitis (Niedergethmann et al, 2007), and urinary tract infection(Olson et al, 2013). Other disorders such as prostate, conjunctiva, and gallbladder have recently been reported by R. planticola
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