Abstract
Five different staining methods were done on the sheep brain compared and the best method was employed to stain the human brain, Alstons staining method is found to be the best among the five staining method, which shows excellent differentiation of grey and white matter. Alston’s method has low shrinkage percentage and the time taken for this procedure is around 8 hrs 30 min. When compared with the unstained section with the stained section show pronounced grey matter. Learning neuroanatomy with wet brain section is difficult in unstained brain specimens, with the induction of staining method in the nueroanatomical structures will be helpful in differentiating the grey and white matter of brain. With the coronal and axial sections in this study various macroscopic neuroanatomical structures can be studied easily.
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