Abstract
To investigate the molecular mechanism for transforming growth factor beta(1) (TGF-beta(1)) on regulation of connective tissue growth factor (CTGF) gene promoter in pancreatic stellate cells (PSCs). We tried to transfect the passaged 2 approximately 5 PSCs with pCTGF-luc plasmids, which were composed of CTGF promoter and PGL3 vector. And different concentrations of TGF-beta(1) or stimulation time were used to observe the reaction of luciferase activities by the measurement of dual-luciferase assay system. TGF-beta(1) could enhance the activities of pCTGF-luc in PSCs by means of time and dose dependently. TGF-beta(1) could stimulate the expression of CTGF gene promoter at the high level in a short time. TGF-beta(1) could enhance the activities of CTGF promoter in PSCs.
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