The Study of Proportion and Molecular Characterisation of Helicobacter pylori in Dyspeptic Patients in Sri Manakula Vinayagar Medical College and Hospital

Abstract

Background: Helicobacter Pylori is the colonized in humans over 100,000 years, the prevalence of H.Pylori in different in various parts of the world. Complications of the organism are varies. This study helps in proportion of H.pylori in the patients attending in tertiary hospital. Aims: 1. The study of proportion of Helicobacter pylori in dyspepsia. 2. Molecular characterisation of Helicobacter pylori in dyspeptic patients in Sri Manakulavinayagar Medical College and Hospital. Methodology: A Hospital based cross section study conducted in Department of General Medicine. The study period was about one and half year after obtaining the ethical committee approval. The study population is of 95 who are came with the complaint of dyspepsia diagnosed by Rome category, patients underwent upper gastro endoscopy tissue sample obtained sent for histopathology staining, Urease test, PCR test. Results: Among the study participants, histopathology report for H.pylori positive for 19 out of 95 participants, percentage 20.0%.Urease report is positive for 22 out of 95 participants, percentage 23.16%.Among the histopathology positive for H.pylori in study participants, PCR for VacA Gene is positive in 10 out of 19 study participants, percentage 52.63%. P valve = 0.001 in Histopathology report, P valve = 0.003 in Urease report, P valve = <0.01 in PCR report. P value <0.05 will be considered statistically significant. Conclusions: H.pylori infections cause gastro-duodenal ulcers, carcinoma, MALT lymphoma. Identifying and treating the infection is important. Patients with VacA gene positive in this study can be treated with four drug regimens.