The Study of Prevalence of Candida Species in Oral Cavity between Habitual and Non-Habitual of Gutka Chewers in Vitro Condition

Abstract

Candida is the most common yeast existing as normal flora of oral cavity. Sometime Candida yeast can cause diseases in the oral cavity. Immune system is weakened due to widespread use of antibiotic, immunosuppressive drugs, age, genetic, malnutrition and hormone disorder as well as HIV infection. Immunocompromised person are more sensitive Aim of the study was to evaluate the prevalence of Candid yeast in those persons that are habitual of chewing gutka and non-habitual. For this study, 450 oral rinse samples were collected aseptically in sterile test tube. These persons visited in dental OPD, Kalka dental college, Meerut. Groups were divided on the bases of habitual and non-habitual of gutka chewer and further clinical history of patients was recorded such as age, gender and socioeconomic status. Each sample were diluted and inoculated on Sabouraud’s Dextrose Agar medium by spread culture technique. The samples were further processed for colony characterization and biochemical test to identify Candida species. As well as measurement of pH level of the oral rinse sample were recorded with pH strip method. 414 isolates were collected from 450 samples, eight Candida species were isolated. Candida albicans were observed as dominant yeast, showed creamy colored, smooth colonies on Sabouraud’s dextrose agar medium. And observed germ tube test, psuedohyphae chlamydospore and various sugars fermentation as well as some other Candida yeasts were also isolated.