The Study of Potentially Lignocellulolytic Actinobacteria Pseudonocardia sp. AI2.

Abstract

The search for microorganisms with potential for bioconversion of lignocellulose is now of immediate interest. Industrial waste is a source of various microorganisms. This paper describes results of the research of potentially lignocellulolytic actinobacteria isolated from activated sludge of the wastewater treatment plant of a pulp and paper mill located in Komi Republic (Russia). One strain of actinobacteria, AI2, was found to be sufficiently active in terms of degradation of lignocellulose-containing materials. Testing of the AI2 isolate demonstrated its ability to synthesize cellulase, dehydrogenase and protease to various extents. The AI2 strain was found capable of biosynthesizing cellulase to 5.5 U/ml. In case of solid-phase fermentation using treated softwood and hardwood sawdust, the content of main components changed most significantly in aspen sawdust: from initial concentration of 20.4% down to 15.6% for lignin, and from 50.6% down to 31.8% for cellulose. In case of liquid-phase fermentation, the content of lignin components decreased significantly in the treated aqueous medium that contained lignosulfonates: from initial concentration of 3.6 g down to 2.1g. Taxonomic study of the AI2 strain of actinobacteria confirmed that it belongs to the rare Pseudonocardia genus of actinomycetes. Based on the results of 16S rRNA sequencing, the AI2 strain is most similar to the species Pseudonocardia carboxydivorans.