The study of planting area on components of Baishouwu anti-tumor effective fraction byRP-HPLC

Abstract

Baishouwu glycosides (CGB) is an anti-tumor effective fraction that has been extracted and isolated from Cynanchum auriculatum Royle ex Wight. To study the differences on components of CGB from 3 main planting areas, we extracted materia medica, isolated and purified CGB from the extract. Physical and chemical identification of CGB indicated that components in CGB had 2-deoxysugar steroids. The results of TLC proved that CGB of different planting areas had similar physical and chemical characteristics. However, the analytical results of RP-HPLC showed that the constituents of CGB from Gansu differed entirely from CGB produced Jiangsu and Shandong. In vitro, MTT results showed that CGB from Gansu had a strong effect on cytotoxicity; however, CGB from Jiangsu and Shandong almost had no effect of cytotoxicity. Constitutes of CGB from Gansu differed entirely from CGB produced Jiangsu and Shandong, this might induce the effect of greatly varied cytotoxicity. Key words: Baishouwu glycosides (CGB), Cynanchum auriculatum Royle ex Wight., anti-tumor effective fraction, tin layer chromatography (TLC), Reversed-phase high-performance liquid chromatography (RP-HPLC), microculture tetrazolium (MTT).