The Study of Physiological Reaction of GPDH in Maize Seedlings Under Abiotic Stress

Abstract

The independent breeding maize variety He No. 344 seeds were used to study the physiological response of glycerol-3-phosphate dehydrogenase (GPDH) on maize under abiotic stresses, including salinity treatment (200 mM NaCl), alkali treatment (150 mM NaHCO3), dehydration treatment (20%-PEG) and low temperature (4°C). The results showed that the abiotic stress can significantly reduced the plant height, root length and dry weight of maize and the combined injury of saline alkali treatment was higher than that of drought and low temperature treatments; The abiotic stress also increased GPDH activity of maize leaf, and the activity of GPDH under saline alkali treatment was significantly higher than that of drought and low temperature treatments; The abiotic stress resulted in increased level of TBARS and H2O2 contents and increased NADH/ NAD+ of maize leaf; Moreover, the abiotic stress also increased SOD, APX, GR and GPX activities and ASA/DHA, GSH/GSSG; In addition, the GPDH activity induced by abiotic stress was significantly correlated with NADH/NAD+, APX, GPX, GR, ASA/DHA, GSH/GSSG, TBARS and H2O2 level, and these results indicate that the increased resistance of maize in the process of abiotic stresses was closely related with the metabolic level of GPDH.