THE STUDY OF PATHOGENIC PROPERTIES OF ENTEROCOCCI ISOLATED FROM THE CARPATHIAN CHEESE

Abstract

The article present the results of the studies pathogenic properties of enterococci isolated from the Carpathian brynza. Due to the high enzymatic activity enterococci give food unique taste properties. However, scientists have an ambiguous attitude to them, primarily due to the acquisition of resistance to antimicrobials by these microorganisms, and the ability of certain species to cause various diseases in humans and animals.
 In the determining of infectivity was found that the intra-gastric administration of live enterococci at a dose of 108 CFU per animal, and in the study of toxicity - inactivated microorganisms, white mice remained alive. During the 14-day of observation period, the laboratory animals were active, had a satisfactory appetite, and maintained reflex excitability for sound and light stimulus. Therefore, the studied strains of enterococci E. durans SB6, E. faecium SB 12, E. durans SB18, E. durans SB20 are not infectious and non-toxic, as they did not cause any clinical symptoms and abnormalities in the overall condition of white mice.
 The tested strains of enterococci don’t have enzymes which are related to the factors of pathogenicity, in particular, they did not have the catalase activity, which have pathogenic aerobic and facultative anaerobic microorganisms, did not produce lecithinase, which causes the de-polymerization of cell membranes of the microorganisms. In addition, the tested microorganisms did not produce plasma-coagulase and fibrinolysin that indicate on the apatogenic properties of the studied microorganisms.
 The tested strains of enterococci did not cause an irritating effect on the mucous membrane of the eye. In the determination of acute toxicity, it was found out that intra-gastric administration of the tested strains did not cause signs of acute poisoning, death and diseases of laboratory animals. At the same time, during the 14-day of observation period, no changes in the clinical condition were detected in the animals.
 In long-term use of enterococci, for 28 days, no changes in the clinical condition of laboratory animals were detected, but observed an increase in body weight of the animals. In particular, on the 28th day of the experiment was noted an increase in body weight of the animals which were administered strains of E. durans SB6, E. faecium SB 12, E. durans SB18, E. durans SB20, respectively, on 7.7, 7.5, 7, 32 and 10.2 %, compared to the values of animals of the control group.