The study of live and frozen marine fish in commodities imports using conventional and the Polymerace Chain Reaction (PCR)

Abstract

Indonesia has big potency of fishery which has entered in export-import market. That export-import activitiy isn’t balance with quality warranty and the quality assurance. The quality warranty and the quality assurance are important because there found bacteria which have zoonotic characteristic. The method of data collection consisted of primary data and secondary data. Data collection was carried out with active participation, observation, interview and literature. Conventional method was used for bacterial examination on a live fish. The use of this method started from the initial isolation, purification, base test, biochemical test and the last was identification process. Bacteria found in a live fish by using the conventional method are Pasteurella haemolytica, Acinetobacter sp, Aeromonas hydrophila, Pseudomonna maltophilia, Pseudomonas sp, Vibrio sp, Pseudomonas mallei, Plesiomonas sp, Alcaligenes sp and Pseudomonas aeruginosa. Polymerace Chain Reaction (PCR) method was used for bacterial examination in frozen fish. This method had many steps, there were extraction, amplification, electrophoresis, and the last was reading the result with UVdoc machine. Primer which was provided that was used to diagnostic DNA bacteria of Aeromonas salmonicida and Edwardsiella tarda. During the study no bacteria of Aeromonas salmonicida and Edwardsiella tarda found, in frozen fish by PCR method.