The study of immune phenotype and function of dendritic cells from hepatitis B virus correlated liver transplant recipients before operation

Abstract

Objective To investigate the immune Phenotype and function of monocyte-derived dendritic cell (DCs) cultured in vitro from hepatitis B virus (HBV) correlated liver transplant (LT) recipients who were in the situation before operation.Methods The DCs of healthy control group and HBV correlated LT recipients before operation were induced by DC culture medium respectively,and then incubated with hepatitis B virus surface antigen (HBsAg) and hepatitis B virus core antigen (HBcAg) to induce matured DCs.Compared the DC morphology and Phenotype and Proliferative capacity and the concentration of Interleukin (IL)-12 and IL-10 in supernatants in each group (control group n =7,patient group n =9),Meanwhile compared the capacity of DCs induced polarization of T cell between incubated group and non-incubated group.Results The rate of CD83 was (53.10 ± 16.23) in patient group,(81.91 ± 9.20) in control group respectively (P ＜0.01).Meanwhile,the difference among HLA-DR and CD40/ CD80/CD86 were not significant (P ＞ 0.1).The proliferative capacity of DC were reduced in LT recipients (P ＜0.05).There was no significant difference in the concentration of IL-12 and IL-10 were (141.94 ± 58.81) ng/L and (12.86 ± 5.72) ng/L in patient group respectively.The rate of Th1 induced by DCs was (7.59 ± 2.62) in non-incubated group,and (11.17 ± 1.11) in incubated group respectively (P ＜ 0.05).Conclusion The immune phenotype and function of DCs were inhibited in LT recipients before operation,but it could induce Th1 response efficiently after incubated with HBsAg and HBcAg in vitro,and increased active immunity. Key words: Hepatitis B virus; Liver transplantation; Dendritic cell; Hepatitis B surface antigen; Hepatitis B core antigen