The study of genetic distinctions of brown Swiss cattle breed with using STR-markers

Abstract

The study of allele pool condition and genetic diversity of Brown Swiss cattle breed was conducted with using STR-markers. Sample collection included samples (n=347) of five breeds: Russian selection Brown Swiss (BSH1), German selection Brown Swiss (BSH2), Jersey (DJ), Simmental (SIM), Kostromskaya(KOS) and Holstein (HOLSH). Polymorphism of 11 microsatellite loci was studied on 16-channell genetic analyzer ABI3130xl. A total of 393 allales with minimal number in DJ group (54) and maximal in SIM group (84) were founded. The number of alleles per locus ranged from 25 (BM1824) to 50 (TGLA122) and average number of alleles per locus ranged from 4.33 (TGLA126) to 8.33 (TGLA122) with a mean of 5.95. Effective number of alleles per locus varied from 2.88 (DJ) to 3.76 (BSH2). Maximal (4.64) and minimal (3.73) numbers of informative alleles were found in BSH2 and BSH1 groups, respectively. Observed heterozygosity range exceeded 0.70 in all groups, except DJ. A total of 23 private alleles were detected ranged from 0,005 (SIM) to 0,385 (DJ). AMOVA analysis showed that 80.068% of variation was observed within populations while 5.186% of variability was intergroup differences. The population structure analysis showed a high level of belonging of all groups to their own cluster. The FCA method revealed an overlapping of multilocus genotypes of BSH1, BSH2 иKOS groups. The results we obtained reveal a high level of genetic diversity in Russian population of Brown Swiss cattle breed.