Abstract
The saliva of ticks contains numerous bioactive molecules with anti-hemostatic and immunomodulatory properties. Due to their abilities of self-renewal and pluripotency, stem cells hold considerable promise in the regenerative medicine and biomedical fields. The present study examines the viability and proliferation of mouse embryonic stem cells (mESCs) following the addition of tick salivary gland extracts obtained from three tick species (Dermacentor marginatus, Rhipicephalus bursa and Hyalomma marginatum) to the mESC medium in different quantities (0.2, 2, 20, 40, 80, and 160 µg/ml). On days 2, 5 and 7 of the treatment, the vitality and proliferation of the cells were determined with CellTiter-Glo and morphological tests. The results showed that the culture supplemented with D. marginatus salivary gland extract at a concentration of 80 µg/ml positively affected the proliferation rate of mESC. It was further shown that all concentrations of the salivary gland extracts derived from H. marginatum and R. bursa had a negative effect on the proliferation rate of mESC when compared to the controls.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
