Abstract
The effects of the dosage of enzyme, liquid/solid ratio, reaction temperature, PH value and reaction time on enzymolysis and saccharification of bagasse pith were studied. The results showed that the optimum conditions of enzymatic hydrolysis of cellulase were PH 5.0,the dosage of enzyme 0.005g/g (oven dry pulp), liquid/solid ratio 35:1, 50°C and s 60h. The changes of bagasse pith during reacting were analyzed through HPLC and FIRT, it were found that the content of xylose increased and improve the yield of reducing sugar in hyrolysis by double-enzymatic(cellulase and lxyanase). The results of FTIR analysis showed that new chemical construction and functional groups were not generated.
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