Abstract
Toxoplasma IgM antibody (Tp-IgM) was measred using Indirect Hemagglutination test (IHA), Latex Hemagglutination Test (LA) and Enzyme-linked Immunosorbent Assay (ELISA) for the purpose of the serological diagnosis of acute toxoplasmosis. 3371 sera obtained in Nagasaki city from September 1979 to July 1982 were examined. In IHA and LA method, the strict examination using Surcose gradient centrifugation, Dye test and Absorption test by Toxoplasma gondii revelaed that only two specimens of one patient contained Tp-IgM exactly. Therefore it seemed that IHA and LA method were not enough to detect Tp-IgM. So we developed new method, that is IgM TOXO-ELISA system, utilizing IgG TOXO ELISA Test kit (M. A. Bioproducts). Protein A treatment serum was used as a specimen and Anti human IgM Al-phos. conjugate was substituted for Anti human IgG Al-phos. conjugate. This method was shown to be extremely more sensitive and specific than IHA and LA method. So it will come into wide use of future.
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Schedule a callMore From: Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases
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