The study of cell implantation in degradable vessel scaffold

Abstract

Objective The degradable artificial material—poly(lactic-co-glycolic acid) (PLGA) was used as the scaffold of the tissue engineering blood vessel for cells implantation,and the better requirement for cell culture was studied.These results may be useful to the manufacture of small diameter tissue engineering blood vessel used in clinical in future.Methods The nonwoven mesh degradable PLGA was used as tissue engineering scaffold.The smooth muscular cell(SMC) and endothelial cell (EC) harvested from fetus umbilical blood vessel were collected as the seeding cells.The SMC and EC were cultivated for 4 weeks respectively in vitro.Then,the SMC was seeded on the surface of the PLGA 4 weeks later,the EC was seeded on the surface of the SMC layer and PLGA.After another 4 weeks,the artificial blood vessel segment with seeding cells was examined in the cellular quality and activity.Results By immunohistochemical method,all test expressions were positive in staining smooth musle actin,yon Willebrand 、CD31 and CD34.By radioimmunoassay,the levels of endothelin and 6-Keto-PGF1 a in PLGA cell culture solution were higher than in control soulture (P ＜ 0.05).These demonstrated that all cells seeded on PLGA still have cellular activity.All seeding cells showed the typical SMC or EC morphological appearances in light microscopic and scanning electric microscopic analyses.Conclusion The results show that the SMC and EC from umbilical vessel,after being cultivated in vitro,as the seeding cells seeded sequentially on the degradable blood vessel scaffold (PLGA),still possess the cellular activity.This may be useful in further studying the small diameter tissue engineering blood vessel. Key words: Blood vessel prosthesis; Umbilical cord; Infant,newborn; Cell culture techniques