Abstract
With the great progress of hemoglobin-based blood substitutes,however, theyallhave a same problem that is hard to get effective stable structure of hemoglobin.The hemoglobin can be oxidized into met hemoglobinin the process of purification,lead to the loss of the function of carrying and transporting oxygen. The combination of CO and placenta blood hemoglobin ability is far higher than that of O2, the structure and properties of carbon monoxide hemoglobin (HbCO) has more stable.Red blood cell (RBC)is mixed with organic solvent for hemolysis and centrifuged for removal of stroma.To prevent MetHb formation during the procedure, Hb was carbonylated in advance.Excessive carbon monoxide pumped to hemoglobin,hemoglobin is converted intoHbCO. HbCO is decarbonylated to regenerate HbO2 by exposing the solution to oxygen and visible light, the content of HbCOand HbCOis measured by blood gas analyzer.By comparing the liquid membrane and film boiling method, determination of oxygenation results.Measured liquid membrane oxygenation ismore effective than hollow fiber module method.Through the liquid membrane method, large quantities of hemoglobin can be treated in production,it also can reduce the loss of hemoglobin during purification,increase oxygen affinity.
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