The study of an acute toxicity of extracts from Veronica teucrium L. and from Veronica longifolia L.

Abstract

The aim of our work were to study the acute toxicity of extracts obtained from herb, leaves, flowers of V. teucrium L. and from herb of V. longifolia L.Materials and methods. The study of the acute toxicity of extracts obtained from herb (Vt-h-w), leaves (Vt-l-chl), flowers (Vt-fl-chl) of V. teucrium L. and herb of V. longifolia L. (Vl-h-sp) was carried out in accordance with International and domestic requirements for humane attitude towards animals. The study was carried out with white, nonlinear, sexually mature, standardized on the physiological and biochemical parameters male mice, divided into 5 groups in 6 animals in each. The investigated extracts were administered intragastrically at a dose of 5000 mg/kg. The assessment of acute toxicity was carried out according to the general condition, dynamics of body mass, calculation of fatal cases of experimental animals, hematological and biochemical parameters. For confirmation of extracts non-toxicity, the histological study of internal organs samples was performed by 14 days from the beginning of the experiment. The class of toxicity was determined by the classification of K. K. Sidorov.Results. The intragastric administration of extracts from V. teucrium L. and from V. longifolia L. in a dose of 5000 mg/kg is not accompanied by the death of experimental animals and changed in their behavior, which indicates the absence of significant toxic effects of substances. The general condition, dynamics of body mass and other parameters were satisfactory and didn’t differ from parameters of intact animals. The investigated extracts didn’t influence to hematological and biochemical parameters of intact animals, and were within the limits of parameters of intact animals. Pathohistological changes in the structure of internal organs (myocardium, lungs, stomach, liver and kidneys) under the influence of the investigated substances were not detected.Conclusions. Significantly cases of an intoxication of animals were not registered, the general condition, dynamics of body mass and other parameters were satisfactory and didn’t differ from parameters of intact animals. Hematological and biochemical parameters were within the limits of parameters of intact animals. Pathohistological changes in the structure of internal organs were not detected. According to the classification of K. K. Sidorov extracts are classified as the IV class of toxicity: low toxicity substances (LD50 = 500–5000 mg/kg).