Abstract
Objective To investigate AGO1 expression,functions and prognostic value in Hepatocellular carcinoma patients underwent radical resection.Methods AGO1 expression was measured in HCCLM3,MHCC97L,and HepG2 cell lines.Using small interfering RNA,AGO1 functions and its effects on p53 were investigated in HCCLM3 and HepG2 cell lines.Immunohistochemistry in tissue microarrays consisting of 200 tumor tissue from hepatocellular carcinoma (HCC) patients underwent radical resection was used to analyze the AGO1 expression with prognosis.Results AGO1 mRNA expression in HCC cell lines HCCLM3,MHCC97L and HepG2 is 0.400 ± 0.025,0.188 ± 0.022 and 0.154 ± 0.019,increased in parallel with the metastatic potential of the HCC cell lines (P ＜ 0.05).Cell proliferation,invasion,and p53 expression significantly decreased after AGO1 depletion in the HCC cell lines.Intratumoral AGO1 was correlated with younger age (P ＜ 0.05),higher α-fetoprotein (P ＜ 0.01),HBeAg-positive status (P ＜0.05),liver cirrhosis (P ＜ 0.05),intrahepatic and extrahepatic recurrence (P ＜ 0.01),and was an independent risk factor for overall survival (P ＜ 0.01) and recurrence-free survival (P ＜ 0.01).Conclusion These findings indicate that AGO1 may promote HCC proliferation and metastasis through the P53 pathways,and AGO1 may be a reliable prognostic factor in HCC patients after resection. Key words: AGO1 ; Hepatocellular carcinoma; Prognosis; p53
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