Abstract

The effects of process variables on the volumetric liquid phase mass transfer coefficient of oxygen, k L a, in a stirred bioreactor were investigated by the static gassing-out method. The process variables chosen were impeller speed, air flow rate, temperature, viscosity and loading (%) of biomass support particles (BSP). The k L a value was obtained as 2.766 min −1 in distilled water at process variables such as stirrer speed 300 rpm, air flow rate 0.3 l min −1, temperature 37°C and pH 7. By using the same process conditions, k L a values obtained in glycerol (50%)+distilled water, distilled water containing BSP (loading 25%, particle volume 0.1625 cm 3) and glycerol (50%)+distilled water containing BSP (loading 25%, particle volume 0.1625 cm 3) were 1.383, 0.933 and 0.450 min −1, respectively. The exponents representing dependence of k L a on the process variables were also obtained by using oxygen absorption data, then these values were compared with literature values.

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