The studies on monoclonal antibody SZ-39 against human glioma cells

Abstract

A hybridoma cell line SZ-39 secreting monoclonal antibody against the human glioma cell has been established by a fusion between NS-1 myeloma cells and spleen cells from mice immunized with human glioma cell lines. Monoclonal antibody (McAb) SZ-39 was analyzed by ELISA, quantitative absorption, indirect immunofluorescence and ABC immunohistology. McAb SZ-39 strongly bound to 9/10 glioma cell lines, 17/20 glioma tissues, weakly bound to one liver cancer cell line and 1/2 lung cancer line, but they did not band with other tested human cancer linse. NcAb SZ-39 have no crossreaction with lymphocyte, ABC red blood cells, white blood cells, blood platelet normal bone marrow cells, fibroblast cells and 12 normal human tissues. The result indicated the antigen recognized by McAb SZ-39 may be a glioma-associated antigen (GAA). This GAA was analyzed by means of Western blotting. It was a MW 180 Kd glycoprotein. The131I-McAb SZ-39 specifically localized in human glioma xenografted in nude mice that indicate it may be useful in radioimmunoimaging and as a target for immunotherapy on human glioma.