Abstract
Platelet Rich Plasma (PRP) works to recruit growth factors, anti-inflammatory mediators, and blood supply to an area that may not heal well under normal conditions (e.g., joints, tendons, ligaments). Previous research has demonstrated that various PRP systems create vastly different final products. The objective of this study was to evaluate the cellular composition of the final plasma product using the Stryker RegenKit system in dogs. A peripheral blood sample was obtained from ten healthy adult dogs and compared to the final plasma product. A standard CBC was performed prior to testing and an additional sample was processed according the manufacturing guidelines for obtaining PRP (10 ml total blood). Comparisons of mean platelet count, erythrocyte count, and leukocyte count were made between the peripheral blood sample and the final plasma product. Results showed that there was no significant difference in platelet count between the peripheral blood CBC and final PRP CBC (p = 0.349). There were significantly fewer erythrocytes and leukocytes in the final plasma product when compared with peripheral blood (p = < 0.0001, p = 0.0318, respectively). These results indicate that the Stryker Regenkit PRP Kit decreases leukocyte and erythrocyte concentrations but does not consistently change platelet concentrations in its final plasma product. This study, in combination with results from similar studies, may allow clinicians to better choose the appropriate PRP system to treat various musculoskeletal conditions in dogs.
Highlights
There has been a growing interest by both pet owners and clinicians in regenerative medicine, platelet rich plasma
The results showed that not all final plasma products had higher platelet concentrations compared to the screening CBC for the same subject
There was no significant difference in concentration of platelets when peripheral blood was compared to the final plasma product of the Stryker system
Summary
There has been a growing interest by both pet owners and clinicians in regenerative medicine, platelet rich plasma. The benefit of PRP administration is attributed to the high concentration of growth factors contained within the alpha granules released when platelets are activated. These alpha granules release a variety of growth factors, including but not limited to, platelet-derived growth factor, insulin-like growth factor, transforming growth factor-β1 and β2, vascular endothelial growth factor, basic fibroblastic growth factor, and epidermal growth factor [10,11,12]. It is generally accepted that there are four classifications of Platelet Rich Plasma [13]. These can be described as: Pure PRP or leukocyte-poor platelet rich plasma (P-PRP), Leukocyte- and Platelet-rich Plasma (L-PRP), Pure Platelet-Rich Fibrin or Leukocyte-Poor
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