Abstract
The morphology and ultrastructure of 48 primary tumours established from 5 cell lines of adult mouse salivary gland epithelial cells transformed in vitro are described. Tumours from 4 of the cells lines were adenocarcinomas with a wide range of structural variation, and resembled human salivary gland carcinomas. The fifth cell line produced tumours with carcinomatous and sarcomatous elements.
Highlights
Epithelial cell foci developed in the cultures
Five tumour-producing cell lines were established from these foci, 4 after treatment of cells with DMBA, and one which arose without carcinogen treatment
A total of 48 primary tumours were established from early passage cells of the 5 cell lines
Summary
Explant cultures of adult mouse salivary gland were treated for 24 h with 7,12-dimethylbenzanthracene (DMBA) on Day 3 of culture. Epithelial cell foci developed in the cultures. Five tumour-producing cell lines were established from these foci, 4 after treatment of cells with DMBA, and one which arose without carcinogen treatment. Details of treatment and tissue-culture techniques are given in an earlier paper (Knowles and Franks, 1977). Cells were removed from their containers with 0-25% Pronase (Calbiochem Limited, London) and 1 or 2 x 106 cells in 0.1 ml of tissue-culture medium were inoculated s.c. into weanling syngeneic hosts. A total of 48 primary tumours were established from early passage cells of the 5 cell lines. Tissues from tumours were taken for histology.
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