The structure of the [formula omitted] pyruvate dehydrogenase complex is probably not unique

Abstract

Measurements of apparent diffusion coefficients of the pyruvate dehydrogenase complex from E. coli , with the use of the active enzyme centrifugation method at different angular velocities, show that the complex is heterogeneous although the heterogeneity is limited. Conditions of extraction and conditions of centrifugation in which the complex would show a monodisperse behaviour have not been found.